An application No. 201611021530 dated June 23, 2016 entitled “A sampling apparatus for in situ volatile collection” invented by ICAR-VPKAS, Almora has been granted patent No. 373714. The inventors of this sampling apparatus are Drs. J. Stanley, S.K. Jain and A. Pattanayak.
Scientists face difficulty in collection of insect pheromones particularly from such insects which do not produce pheromones in captivity or on disturbance. This invention particularly relates to in-situ headspace sampling apparatus for collection of volatiles directly without having an entrainment chamber for entraining the volatiles releasing sample. The apparatus of the present invention is adapted for onsite collection of volatiles which are actively released by biological materials without affecting quality and quantity of the volatiles. The apparatus of the present invention is portable and intended to collect volatiles in-situ conditions. The volatiles thus collected are not only used to study the biology and communication pattern of organisms but also in biodiversity monitoring, pest control etc.
The apparatus consists of at least one receptor with tapering end, one or more sorbent traps with single or branched stem where the adsorbent material is being positioned within, a flexible tube for connecting sorbent trap(s) to suction creating mechanism and a suction creating mechanism at the end. The sorbent trap is a conical tubular structure detachably positioned in the tapering end of the receptor. The apparatus was successfully utilized to collect the pheromone of scarab beetle/ chafer beetle/ white-grub beetle, Holotrichia seticollis (Coleoptera: Scarabaeidae: Melolonthinae). Holotrichia seticollis is one of the predominant white-grub species in Uttarakhand (Almora) but do not get attracted to light. This less-phototactic nature of H. seticollis makes light trap not effective for its management. However, the pheromone extracted from H. seticollis using the above apparatus is found effective in trapping H. seticollis males in the field aid in pest management.